Composite

Part:BBa_K1162307:Design

Designed by: Ryan Putman   Group: iGEM13_Utah_State   (2013-09-25)


WAM-1 antimicrobial peptide fused with 8x spider silk protein, with 10x His-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2562
    Illegal XhoI site found at 2463
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RFC 23. ATG (Met) at start of coding region.


Source

Composite of genes from tammar wallaby and Argiope aurantia spider

References